Position-dependent impact of hexafluoroleucine and trifluoroisoleucine on protease digestion

• Susanne Huhmann,
• Anne-Katrin Stegemann,
• Kristin Folmert,
• Damian Klemczak,
• Johann Moschner,
• Michelle Kube and
• Beate Koksch

Beilstein J. Org. Chem. 2017, 13, 2869–2882, doi:10.3762/bjoc.13.279

• recognition site is predominantly hydrophobic and there is evidence that not the sequence of the substrate is of importance in the recognition but only the volume of the side chains [84]. Substitution of Ala in position P2 with Ile and Leu leads to a greater or comparable amount of degradation after 120 min

• proteolytic activity [79]. Its active center contains an extended binding region consisting of several subsites, at least four or five subsites on the N-terminal side of the scissile bond (S1 to S4/S5) and three subsites C-terminal to the scissile bond (S1’ to S3’) [81][82][83]. The “bottom” of substrate

Radical polymerization by a supramolecular catalyst: cyclodextrin with a RAFT reagent

• Kohei Koyanagi,
• Yoshinori Takashima,
• Takashi Nakamura,
• Hiroyasu Yamaguchi and
• Akira Harada

Beilstein J. Org. Chem. 2016, 12, 2495–2502, doi:10.3762/bjoc.12.244

• distributed polymers. In the presence of 1,6-hexanediol (C6 diol) which works as a competitive molecule by being included in the α-CD cavity, the reaction yield was lower than that without C6 diol. Keywords: cyclodextrin; radical polymerization; RAFT polymerization; substrate recognition site; supramolecular

Active site diversification of P450cam with indole generates catalysts for benzylic oxidation reactions

• Paul P. Kelly,
• Anja Eichler,
• Susanne Herter,
• David C. Kranz,
• Nicholas J. Turner and
• Sabine L. Flitsch

Beilstein J. Org. Chem. 2015, 11, 1713–1720, doi:10.3762/bjoc.11.186

• interactions [23], whereas Met184 is part of the P450cam substrate recognition site 2 (SRS 2) [24]. Accordingly, the entire P450cam active site was partitioned into seven residue pairs which were targeted in site-directed mutagenesis experiments in the manner of CASTing (Figure 1) [25]. NDT codon degeneracy